Identification of mouse ZP3 protein in mammalian oocytes with antisera against synthetic ZP3 peptides.

@article{Hinsch1994IdentificationOM,
  title={Identification of mouse ZP3 protein in mammalian oocytes with antisera against synthetic ZP3 peptides.},
  author={K D Hinsch and Elvira Hinsch and B. Meinecke and Edda T{\"o}pfer-Petersen and Simon G Pfisterer and W. B. Schill},
  journal={Biology of reproduction},
  year={1994},
  volume={51 2},
  pages={193-204}
}
The mouse zona pellucida (ZP) protein ZP3 plays an important role in the process of fertilization by mediating sperm binding and the acrosome reaction. ZP3 primary structures are highly conserved, as revealed by cDNA cloning. We raised antisera against synthetic peptides that are either conserved in the structure of ZP3 from different mammalian species (AS ZP3-5 and AS ZP3-6) or specific for mouse ZP3 (AS ZP3-2). In ovary sections, AS ZP3-2 revealed immunoreactivity only to mouse ZP. AS ZP3-5… CONTINUE READING