Identification of mosquito avian-derived blood meals by polymerase chain reaction-heteroduplex analysis.

@article{Lee2002IdentificationOM,
  title={Identification of mosquito avian-derived blood meals by polymerase chain reaction-heteroduplex analysis.},
  author={Joon Hak Lee and Hassan K. Hassan and Geoff B. Hill and Eddie W Cupp and Tarig B. Higazi and Carl Mitchell and Marvin S. Godsey and Thomas R. Unnasch},
  journal={The American journal of tropical medicine and hygiene},
  year={2002},
  volume={66 5},
  pages={599-604}
}
A polymerase chain reaction (PCR) heteroduplex assay (HDA) was developed to identify avian derived mosquito blood meals to the species level. The assay used primers amplifying a fragment of the cytochrome B gene from vertebrate but not invertebrate species. In Culex tarsalis fed on quail, PCR products derived from the quail cytochrome B gene were detected seven days post-engorgement. In an analysis of wild-caught mosquitoes, 85% of blood-fed mosquitoes produced detectable PCR products… CONTINUE READING
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Identification of blood meals in hematophagous Diptera by polymerase chain reaction amplification and heteroduplex analysis

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