Identification of molecular vulnerabilities in human multiple myeloma cells by RNA interference lethality screening of the druggable genome.

@article{Tiedemann2012IdentificationOM,
  title={Identification of molecular vulnerabilities in human multiple myeloma cells by RNA interference lethality screening of the druggable genome.},
  author={Rodger E Tiedemann and Yuan Xao Zhu and Jessica Young Schmidt and Chang Xin Shi and Chris Sereduk and Hongwei Yin and Spyro Mousses and A Keith Stewart},
  journal={Cancer research},
  year={2012},
  volume={72 3},
  pages={757-68}
}
Despite recent advances in targeted treatments for multiple myeloma, optimal molecular therapeutic targets have yet to be identified. To functionally identify critical molecular targets, we conducted a genome-scale lethality study in multiple myeloma cells using siRNAs. We validated the top 160 lethal hits with four siRNAs per gene in three multiple myeloma cell lines and two non-myeloma cell lines, cataloging a total of 57 potent multiple myeloma survival genes. We identified the Bcl2 family… CONTINUE READING