Identification of methylation sites and effects of phototaxis stimuli on transducer methylation in Halobacterium salinarum.

@article{Perazzona1999IdentificationOM,
  title={Identification of methylation sites and effects of phototaxis stimuli on transducer methylation in Halobacterium salinarum.},
  author={Bastianella Perazzona and John L Spudich},
  journal={Journal of bacteriology},
  year={1999},
  volume={181 18},
  pages={
          5676-83
        }
}
The two transducers in the phototaxis system of the archaeon Halobacterium salinarum, HtrI and HtrII, are methyl-accepting proteins homologous to the chemotaxis transducers in eubacteria. Consensus sequences predict three glutamate pairs containing potential methylation sites in HtrI and one in HtrII. Mutagenic substitution of an alanine pair for one of these, Glu265-Glu266, in HtrI and for the homologous Glu513-Glu514 in HtrII eliminated methylation of these two transducers, as demonstrated by… CONTINUE READING

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