Identification of membrane proteome of Paracoccidioides lutzii and its regulation by zinc

  • Juliana Santana de Curcio, Marielle Garcia Silva, +4 authors Célia Maria de Almeida Soares
  • Published 2017 in Future science OA

Abstract

Aim During infection development in the host, Paracoccidioides spp. faces the deprivation of micronutrients, a mechanism called nutritional immunity. This condition induces the remodeling of proteins present in different metabolic pathways. Therefore, we attempted to identify membrane proteins and their regulation by zinc in Paracoccidioides lutzii. Materials & methods Membranes enriched fraction of yeast cells of P. lutzii were isolated, purified and identified by 2D LC-MS/MS detection and database search. Results & conclusion Zinc deprivation suppressed the expression of membrane proteins such as glycoproteins, those involved in cell wall synthesis and those related to oxidative phosphorylation. This is the first study describing membrane proteins and the effect of zinc deficiency in their regulation in one member of the genus Paracoccidioides.

DOI: 10.4155/fsoa-2017-0044

Cite this paper

@inproceedings{Curcio2017IdentificationOM, title={Identification of membrane proteome of Paracoccidioides lutzii and its regulation by zinc}, author={Juliana Santana de Curcio and Marielle Garcia Silva and Mirelle Garcia Silva Bail{\~a}o and S{\^o}nia Nair B{\'a}o and Luciana Casaletti and Alexandre Mello Bail{\~a}o and C{\'e}lia Maria de Almeida Soares}, booktitle={Future science OA}, year={2017} }