Identification of melanoma-associated antigens using fixed tissue screening of antibodies.

Abstract

Early culture supernatants from hybridomas that were obtained through fusions of mouse myeloma cells with lymphocytes of melanoma-immunized mice were screened for their reactivity with a paraffin-embedded cell block of a melanoma cell line, using a biotin:avidin immunoperoxidase procedure. Eleven monoclonal antibodies were derived that define several new… (More)

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Cite this paper

@article{Atkinson1984IdentificationOM, title={Identification of melanoma-associated antigens using fixed tissue screening of antibodies.}, author={Bradley J Atkinson and Carl Ernst and Barbara F. D. Ghrist and Marc Herlyn and Maciej Blaszczyk and Ashley A Ross and Dorothee M Herlyn and Zenon Steplewski and Hillary Koprowski}, journal={Cancer research}, year={1984}, volume={44 6}, pages={2577-81} }