Identification of mammal species using species-specific DNA pyrosequencing.

  title={Identification of mammal species using species-specific DNA pyrosequencing.},
  author={Andreas O Karlsson and Gunilla Holmlund},
  journal={Forensic science international},
  volume={173 1},
In forensic casework it is highly relevant to be able to deduce the species origin of an unknown biological sample. For such a purpose we have designed and developed an assay for species identification based on DNA sequencing of two short mitochondrial DNA amplicons. In short, partial 12S rRNA and partial 16S rRNA fragments (approximately 100bp) are amplified by PCR followed by direct sequencing using pyrosequencing technique. Due to properties of the chosen targets, the same PCR conditions and… CONTINUE READING