Identification of major outer surface proteins of Streptococcus agalactiae.

  title={Identification of major outer surface proteins of Streptococcus agalactiae.},
  author={Martin John Glenton Hughes and Joanne C. Moore and Jonathan D. Lane and Rebecca M. Wilson and Philippa K. Pribul and Zabin N. Younes and Richard J. Dobson and Paul Everest and Andrew J. Reason and Joanne M Redfern and Fiona M. Greer and Thanai Paxton and Maria Panico and Howard R. Morris and Robert G. Feldman and J. D. Santangelo},
  journal={Infection and immunity},
  volume={70 3},
To identify the major outer surface proteins of Streptococcus agalactiae (group B streptococcus), a proteomic analysis was undertaken. An extract of the outer surface proteins was separated by two-dimensional electrophoresis. The visualized spots were identified through a combination of peptide sequencing and reverse genetic methodologies. Of the 30 major spots identified as S. agalactiae specific, 27 have been identified. Six of these proteins, previously unidentified in S. agalactiae, were… CONTINUE READING