Identification of mRNAs regulated by interferon-gamma in cultured rat astrocytes by PCR differential display.

@article{Kuchinke1995IdentificationOM,
  title={Identification of mRNAs regulated by interferon-gamma in cultured rat astrocytes by PCR differential display.},
  author={W Kuchinke and Ronald P. Hart and G. Miller Jonakait},
  journal={Neuroimmunomodulation},
  year={1995},
  volume={2 6},
  pages={347-55}
}
We have used RNA differential display to identify specific mRNA species that are regulated by interferon-gamma (IFN gamma) treatment of enriched astrocyte cultures. Following a 24-hour treatment with IFN, mRNA was subjected to differential display using 15 different random primers. 105 upregulated and 5 downregulated cDNAs were selected for further sequencing and identification. Northern blot analysis confirmed the upregulation of 13 genes identical or highly similar to: intercrine… CONTINUE READING
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