Identification of low-Ca2+- and high-Ca2+-requiring neutral proteases in rat retina.

@article{Tsung1985IdentificationOL,
  title={Identification of low-Ca2+- and high-Ca2+-requiring neutral proteases in rat retina.},
  author={P K Tsung and John B. Lombardini},
  journal={Experimental eye research},
  year={1985},
  volume={41 1},
  pages={97-103}
}
Two low-Ca2+-requiring proteases (calpain I) and one high-Ca2+-requiring protease (calpain II) have been separated from the cytosol of rat retina by DEAE-cellulose column chromatography. Calpain I was half-maximally activated at 3 microM free Ca2+ and fully activated at 10 microM free Ca2+. Half-maximal activation of calpain II was at 0.4 mM free Ca2+ while… CONTINUE READING