Identification of leukocyte cationic proteins that interact with ceruloplasmin

  title={Identification of leukocyte cationic proteins that interact with ceruloplasmin},
  author={Alexey V Sokolov and Maria O. Pulina and Kira V. Ageeva and Olga L Runova and Elena T. Zakharova and Vadim B. Vasilyev},
  journal={Biochemistry (Moscow)},
Proteins from leukocytes were investigated for their ability to interact with ceruloplasmin (Cp), a copper-containing glycoprotein of human plasma. Extract from leukocytes was subjected to affinity chromatography on Cp-Sepharose, after which proteins were eluted from the resin with 0.5 M NaCl in Tris-HCl, pH 7.4. SDS-PAGE of the eluate revealed protein bands with molecular weights 78, 57, 40, 30, 16, and 12 kD. Among these, Western blotting detected myeloperoxidase (57, 40, and 12 kD) and… CONTINUE READING