Identification of isoenzymes using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

@article{Hardouin2006IdentificationOI,
  title={Identification of isoenzymes using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.},
  author={Julie Hardouin and Marie Hubert-Roux and Agn{\`e}s F. Delmas and C. Lange},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2006},
  volume={20 5},
  pages={725-32}
}
The identification of isoforms is one of the great challenges in proteomics due to the large number of identical amino acids preventing their separations by two-dimensional electrophoresis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) has become a rapid and sensitive tool in proteomics, notably with the new instrumental improvements. In this study, we used several acquisition modes of MALDI-TOFMS to identify isoforms of porcine glutathiones S… CONTINUE READING