Identification of iron(III) peroxo species in the active site of the superoxide reductase SOR from Desulfoarculus baarsii.

@article{Math2002IdentificationOI,
  title={Identification of iron(III) peroxo species in the active site of the superoxide reductase SOR from Desulfoarculus baarsii.},
  author={Christelle Math{\'e} and Tony A. Mattioli and Olivier Horner and Murielle Lombard and Jean-Marc Latour and Marc Fontecave and Vincent Nivi{\`e}re},
  journal={Journal of the American Chemical Society},
  year={2002},
  volume={124 18},
  pages={4966-7}
}
The active site of superoxide reductase SOR consists of an Fe2+ center in an unusual [His4 Cys1] square-pyramidal geometry. It specifically reduces superoxide to produce H2O2. Here, we have reacted the SOR from Desulfoarculus baarsii directly with H2O2. We have found that its active site can transiently stabilize an Fe3+-peroxo species that we have spectroscopically characterized by resonance Raman. The mutation of the strictly conserved Glu47 into alanine results in a stabilization of this Fe3… CONTINUE READING
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