Identification of iodination sites in cytochrome c by high-performance liquid chromatography and fast atom bombardment mass spectrometry.

Abstract

Site-directed covalent modification of proteins is currently used to study the molecular structure of enzyme active sites. Radioactive labels together with protein sequencing by Edman degradation have been previously employed to identify the site of modification. One drawback of such a procedure is that most reagents are not commercially available in… (More)

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