Identification of inhibitors of bacterial transcription/translation machinery utilizing a miniaturized 1536-well format screen.


This report presents the miniaturization of a HTS screen to identify inhibitors of prokaryotic transcription-translation in a 1536-well format. The in vitro assay design utilized the bacterial expression machinery to drive expression of a firefly luciferase reporter gene, which was read as an endpoint luminesence measurement. This multicomponent system… (More)


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