Identification of increased amounts of eppin protein complex components in sperm cells of diabetic and obese individuals by difference gel electrophoresis.

@article{Paasch2011IdentificationOI,
  title={Identification of increased amounts of eppin protein complex components in sperm cells of diabetic and obese individuals by difference gel electrophoresis.},
  author={Uwe Paasch and Falk Heidenreich and Theresia Pursche and Eberhard Kuhlisch and Karina Kettner and Sonja Grunewald and J{\"u}rgen Kratzsch Kratzsch and Gunnar Dittmar and H. -J. Glander and Bernard Hoflack and Thomas M. Kriegel},
  journal={Molecular & cellular proteomics : MCP},
  year={2011},
  volume={10 8},
  pages={M110.007187}
}
Metabolic disorders like diabetes mellitus and obesity may compromise the fertility of men and women. To unveil disease-associated proteomic changes potentially affecting male fertility, the proteomes of sperm cells from type-1 diabetic, type-2 diabetic, non-diabetic obese and clinically healthy individuals were comparatively analyzed by difference gel electrophoresis. The adaptation of a general protein extraction procedure to the solubilization of proteins from sperm cells allowed for the… CONTINUE READING