Identification of in vivo phosphorylation sites on human deoxycytidine kinase. Role of Ser-74 in the control of enzyme activity.

@article{Smal2006IdentificationOI,
  title={Identification of in vivo phosphorylation sites on human deoxycytidine kinase. Role of Ser-74 in the control of enzyme activity.},
  author={Caroline Smal and Didier Vertommen and Luc Bertrand and Sandrine Ntamashimikiro and Mark H. Rider and Eric Van Den Neste and Françoise Bontemps},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 8},
  pages={4887-93}
}
Deoxycytidine kinase (dCK) catalyzes the rate-limiting step of the deoxyribonucleoside salvage pathway in mammalian cells and plays a key role in the activation of numerous nucleoside analogues used in anti-cancer and antiviral chemotherapy. Although compelling evidence indicated that dCK activity might be regulated by phosphorylation/dephosphorylation, direct demonstration was lacking. Here we showed that dCK overexpressed in HEK 293T cells was labeled after incubating the cells with [32P… CONTINUE READING