Identification of important bases for the self-cleavage activity at two single-stranded regions of genomic HDV ribozyme.

Abstract

In order to determine important bases at two single-stranded regions [SSrA (726-731 nt) and SSrB (762-766)] derived mainly from secondary structure models in genomic hepatitis delta virus (HDV) ribozyme possessing self-cleavage activity, we have constructed several point mutants at these two regions on the HDV88 molecule (683-770). Among the bases at SSrA and SSrB regions C763 was found to play an essential role during self-cleavage process since substitutions to any other bases viz. A or G or U completely abolished the activity.

Cite this paper

@article{Nishikawa1992IdentificationOI, title={Identification of important bases for the self-cleavage activity at two single-stranded regions of genomic HDV ribozyme.}, author={Shin Nishikawa and Yeongjun Suh and Penmetcha K R Kumar and Jun-ichi Kawakami and Fumiko Nishikawa and Kazunari Taira}, journal={Nucleic acids symposium series}, year={1992}, volume={27}, pages={41-2} }