Identification of glycosyl hydrolases from a metagenomic library of microflora in sugarcane bagasse collection site and their cooperative action on cellulose degradation.

@article{Kanokratana2015IdentificationOG,
  title={Identification of glycosyl hydrolases from a metagenomic library of microflora in sugarcane bagasse collection site and their cooperative action on cellulose degradation.},
  author={Pattanop Kanokratana and Lily Eurwilaichitr and Kusol Pootanakit and Verawat Champreda},
  journal={Journal of bioscience and bioengineering},
  year={2015},
  volume={119 4},
  pages={384-91}
}
Lignocellulose decomposition is a natural process involving the cooperative action of various glycosyl hydrolases (GH) on plant cell wall components. In this study, a metagenomic library was constructed to capture the genetic diversity of microbes inhabiting an industrial bagasse collection site. A variety of putative genes encoding GH families 2, 3, 5, 9, 11, and 16 were identified using activity-based screening, which showed low to moderate homology to various cellulases and hemicellulases… CONTINUE READING
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