Identification of glutamate 344 as the catalytic residue in the active site of pig heart CoA transferase.

@article{Rochet1994IdentificationOG,
  title={Identification of glutamate 344 as the catalytic residue in the active site of pig heart CoA transferase.},
  author={Jean Charles Rochet and William A. Bridger},
  journal={Protein science : a publication of the Protein Society},
  year={1994},
  volume={3 6},
  pages={975-81}
}
The enzyme CoA transferase (succinyl-CoA:3-ketoacid coenzyme A transferase [3-oxoacid CoA transferase], EC 2.8.3.5) is essential for the metabolism of ketone bodies in the mammalian mitochondrion. It is known that its catalytic mechanism involves the transient thioesterification of an active-site glutamate residue by CoA. As a means of identifying this… CONTINUE READING