Identification of genetic determinants for the hemolytic activity of Streptococcus agalactiae by ISS1 transposition.

@article{Spellerberg1999IdentificationOG,
  title={Identification of genetic determinants for the hemolytic activity of Streptococcus agalactiae by ISS1 transposition.},
  author={Barbara Spellerberg and Birte Pohl and Gerhard Haase and Simone Martin and Josefine Weber-Heynemann and Rudolf Luetticken},
  journal={Journal of bacteriology},
  year={1999},
  volume={181 10},
  pages={3212-9}
}
Streptococcus agalactiae is a poorly transformable bacterium and studies of molecular mechanisms are difficult due to the limitations of genetic tools. Employing the novel pGh9:ISS1 transposition vector we generated plasmid-based mutant libraries of S. agalactiae strains O90R and AC475 by random chromosomal integration. A screen for mutants with a nonhemolytic phenotype on sheep blood agar led to the identification of a genetic locus harboring several genes that are essential for the hemolytic… CONTINUE READING
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