Identification of genes that enhance cellulase protein production in yeast.

  title={Identification of genes that enhance cellulase protein production in yeast.},
  author={Takao Kitagawa and Katsunori Kohda and Kenro Tokuhiro and Hisashi Hoshida and Rinji Akada and Haruo Takahashi and Takao Imaeda},
  journal={Journal of biotechnology},
  volume={151 2},
In order to enhance heterologous cellulase protein production in yeast, a plasmid harboring the endoglucanase gene from Clostridium thermocellum (Ctcel8A) was used to systematically transform a homozygous diploid yeast deletion strain collection. We identified 55 deletion strains that exhibited enhanced endoglucanase activity compared with that of the wild-type strain. Genes disrupted in these strains were classified into the categories of transcription, translation, phospholipid synthesis… CONTINUE READING
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