Identification of genes differentially expressed in association with metastatic potential of K-1735 murine melanoma by messenger RNA differential display.

@article{Hashimoto1996IdentificationOG,
  title={Identification of genes differentially expressed in association with metastatic potential of K-1735 murine melanoma by messenger RNA differential display.},
  author={Y Masayuki Hashimoto and Nobuko Shindo-Okada and Masachika Tani and Ken-ichi Takeuchi and Hiroshi Toma and Jun Yokota},
  journal={Cancer research},
  year={1996},
  volume={56 22},
  pages={
          5266-71
        }
}
To identify genes differentially expressed in association with the metastatic potential of K-1735 mouse melanoma cells, the mRNA differential display method was applied to compare mRNAs from high- and low-metastatic K-1735-derived cells. Three of the high- and three of the low-metastatic clones were used to reduce the false positives in the initial screening, and Southern blot screening against reverse transcription-PCR products was used to confirm that cDNA fragments detect differential… CONTINUE READING

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