Identification of genes associated with natural competence in Helicobacter pylori by transposon shuttle random mutagenesis.

@article{Chang2001IdentificationOG,
  title={Identification of genes associated with natural competence in Helicobacter pylori by transposon shuttle random mutagenesis.},
  author={K C Chang and Y C Yeh and Tian Long Lin and Jianchu Wang},
  journal={Biochemical and biophysical research communications},
  year={2001},
  volume={288 4},
  pages={
          961-8
        }
}
To identify genes involved in DNA transformation, we generated 1500 insertion mutants of a Helicobacter pylori strain by transposon shuttle mutagenesis. All mutant strains were screened for their frequency of natural transformation. A total of 20 mutant strains were found to exhibit a significantly decreased transformation frequency. DNA sequencing revealed seven genetic loci, including the reported comB locus, HP0017 (a putative virB4 homologue) and five loci without database match (HP0015… CONTINUE READING

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