Identification of functionally important amino acid residues in Zymomonas mobilis levansucrase.

  title={Identification of functionally important amino acid residues in Zymomonas mobilis levansucrase.},
  author={Hideshi Yanase and Masahiro Maeda and Eigo Hagiwara and Hiroyuki Yagi and Kenji Taniguchi and Kenji Okamoto},
  journal={Journal of biochemistry},
  volume={132 4},
The catalytic residues of levansucrase (sucrose:2,6-beta-D-fructan 6-beta-D-fructosyltransferase, EC from Zymomonas mobilis were analyzed by random mutation and site-directed mutagenesis. We found that substitution of Glu278 with Asp and His reduced the k(cat) for sucrose hydrolysis 30- and 210-fold, respectively, strongly suggesting Glu278 plays a key role in catalyzing this reaction. Given the likelihood that another acidic amino residue was also involved, we constructed variants in… CONTINUE READING

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