Identification of functional receptors for ciliary neurotrophic factor on neuronal cell lines and primary neurons.

Abstract

The lack of reagents or molecular probes specific for the ciliary neurotrophic factor (CNTF) receptor has hindered characterization of the molecular mechanism(s) by which CNTF influences the proliferation, survival, and differentiation of cells of the vertebrate nervous system. We have developed methods for the detection and separation of cells expressing CNTF receptors by using a variety of binding assays based on a genetically engineered CNTF molecule containing an "epitope tag" at its C-terminus. These assays have allowed us to identify several neuronal cell lines, as well as embryonic and adult neurons in primary cultures, that bind CNTF and functionally respond to CNTF by rapidly activating the transcription of immediate early primary response genes.

Statistics

050'93'96'99'02'05'08'11'14'17
Citations per Year

226 Citations

Semantic Scholar estimates that this publication has 226 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Squinto1990IdentificationOF, title={Identification of functional receptors for ciliary neurotrophic factor on neuronal cell lines and primary neurons.}, author={Stephen P. Squinto and Thomas H. Aldrich and Robert McGregor Lindsay and Donna M Morrissey and N Panayotatos and Salvador Mario Bianco and Mark E. Furth and George D. Yancopoulos}, journal={Neuron}, year={1990}, volume={5 6}, pages={757-66} }