Identification of four structural genes and two putative promoters necessary for utilization of phenanthrene naphthalene, fluoranthene, and by Sphingomonas paucimobilis var. EPA505.

@inproceedings{Story2000IdentificationOF,
  title={Identification of four structural genes and two putative promoters necessary for utilization of phenanthrene naphthalene, fluoranthene, and by Sphingomonas paucimobilis var. EPA505.},
  author={Sandra Paige Story and Stephen Parker and Jon D Kline and Tzuen-rong Jeremy Tzeng and James G. Mueller and Ellis L. Kline},
  year={2000}
}
Abstract Sphingomonas paucimobilis var. EPA505 utilizes fluoranthene (FLA), naphthalene (NAP), and phenanthrene (PHE) as sole carbon sources for energy and growth. A genetic library of EPA505 was constructed using mini-Tn5 promoter reporter genes encoding for tetracycline resistance ( tc p− ) or luminescence ( lux AB p− ). Out of 2250 Tn5 mutants, ten were deficient in utilization of FLA, NAP, and/or PHE as sole carbon sources. Three classes of Tn5 mutants were defined: classI ( nap − phe − fla… CONTINUE READING