Identification of essential genes for cancer immunotherapy

  title={Identification of essential genes for cancer immunotherapy},
  author={Shashank J. Patel and Neville E. Sanjana and Rigel J Kishton and Arash Eidizadeh and Suman Kumar Vodnala and Maggie Cam and Jared J. Gartner and Li Jia and Seth M. Steinberg and Tori N. Yamamoto and Anand S. Merchant and Gautam U. Mehta and Anna Chichura and Ophir Shalem and Eric Tran and Robert L. Eil and Madhusudhanan Sukumar and Eva Perez Guijarro and C Day and Paul F. Robbins and Steven R. Feldman and Glenn Merlino and Fengge Zhang and Nicholas P Restifo},
Somatic gene mutations can alter the vulnerability of cancer cells to T-cell-based immunotherapies. Here we perturbed genes in human melanoma cells to mimic loss-of-function mutations involved in resistance to these therapies, by using a genome-scale CRISPR–Cas9 library that consisted of around 123,000 single-guide RNAs, and profiled genes whose loss in tumour cells impaired the effector function of CD8+ T cells. The genes that were most enriched in the screen have key roles in antigen… CONTINUE READING
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Cancer immunology. Mutational landscape determines sensitivity to PD-1 blockade in non-small cell lung cancer

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