Identification of essential arginines in the acetate kinase from Methanosarcina thermophila.

@article{SinghWissmann2000IdentificationOE,
  title={Identification of essential arginines in the acetate kinase from Methanosarcina thermophila.},
  author={K Singh-Wissmann and Rebecca D Miles and Cheryl Ingram-Smith and James G. Ferry},
  journal={Biochemistry},
  year={2000},
  volume={39 13},
  pages={3671-7}
}
Site-directed mutagenesis is a powerful tool for identifying active-site residues essential for catalysis; however, this approach has only recently become available for acetate kinase. The enzyme from Methanosarcina thermophila has been cloned and hyper-produced in a highly active form in Escherichia coli (recombinant wild-type). The role of arginines in this acetate kinase was investigated. Five arginines (R91, R175, R241, R285, and R340) in the M. thermophila enzyme were selected for… CONTINUE READING

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