Identification of endopeptidase genes from the genomic sequence of Lactobacillus helveticus CNRZ32 and the role of these genes in hydrolysis of model bitter peptides.

@article{Sridhar2005IdentificationOE,
  title={Identification of endopeptidase genes from the genomic sequence of Lactobacillus helveticus CNRZ32 and the role of these genes in hydrolysis of model bitter peptides.},
  author={Vidya R. Sridhar and Joanne E. Hughes and Dennis L. Welker and Jeffery R. Broadbent and James L. Steele},
  journal={Applied and environmental microbiology},
  year={2005},
  volume={71 6},
  pages={3025-32}
}
Genes encoding three putative endopeptidases were identified from a draft-quality genome sequence of Lactobacillus helveticus CNRZ32 and designated pepO3, pepF, and pepE2. The ability of cell extracts from Escherichia coli DH5alpha derivatives expressing CNRZ32 endopeptidases PepE, PepE2, PepF, PepO, PepO2, and PepO3 to hydrolyze the model bitter peptides, beta-casein (beta-CN) (f193-209) and alpha(S1)-casein (alpha(S1)-CN) (f1-9), under cheese-ripening conditions (pH 5.1, 4% NaCl, and 10… CONTINUE READING

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Analysis of promoter sequences from Lactobacillus helveticus CNRZ32 and their activity in other lactic acid bacteria

  • Chen, Y.-S, J. L. Steele
  • J. Appl. Microbiol
  • 2005

Identification and characterization of Lactobacillus helveticus PepO2, an endopeptidase with post-proline specificity

  • Chen, Y.-S, J. E. Christensen, M. Strickland, J. L. Steele
  • Appl. Environ. Microbiol
  • 2003

Impact of cheese defects on U.S. graded cheeses

  • M. Smukowski, W. L. Wendorff, Y. Ping, R. D. Rao
  • J. Dairy Sci
  • 2003

Accelerating cheese proteolysis by altering Lactococcus lactis proteolytic system with lactobacilli peptidases

  • P. Courtin, M. Nardi, +6 authors V. Monnet
  • Int. Dairy J. 12:447–454
  • 2002

Global analysis of the Lactobacillus helveticus CNRZ32 proteolytic enzyme system, abstr. K13

  • J. R. Broadbent, J. L. Steele, D. L. Welker, J. E. Hughes
  • 7th International Symposium of Lactic Acid…
  • 2002

Development of analytical methods to quantify the insoluble and soluble calcium content in Cheddar cheese and a study of its influence on cheese

  • A. Hassan
  • 2001

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