Identification of domains conferring G protein regulation on inward rectifier potassium channels

  title={Identification of domains conferring G protein regulation on inward rectifier potassium channels},
  author={M. T. Kunkel and E. Peralta},
  • M. T. Kunkel, E. Peralta
  • Published 1995
  • Biology, Medicine
  • Cell
  • Cardiac m2 muscarinic acetylcholine receptors reduce heart rate by coupling to heterotrimeric (alpha beta gamma) guanine nucleotide-binding (G) proteins that activate IKACh, an inward rectifier K+ channel (IRK). Activation of the GIRK subunit of IKACh requires G beta gamma subunits; however, the structural basis of channel regulation is unknown. To determine which sequences confer G beta gamma regulation upon IRKs, we generated chimeric proteins composed of GIRK and RB-IRK2, a related, G… CONTINUE READING
    158 Citations
    G-protein mediated gating of inward-rectifier K+ channels.
    • 229
    Single Channel Studies of Inward Rectifier Potassium Channel Regulation by Muscarinic Acetylcholine Receptors
    • 7
    • PDF
    A Switch Mechanism for Gβγ Activation of IKACh *
    • 56
    • PDF
    Single channel analysis of the regulation of GIRK1/GIRK4 channels by protein phosphorylation.
    • 20
    • PDF
    G-Protein Regulation of Channels
    • 2


    Recombinant G-protein beta gamma-subunits activate the muscarinic-gated atrial potassium channel.
    • 103
    • Highly Influential
    A region of adenylyl cyclase 2 critical for regulation by G protein beta gamma subunits.
    • 257
    Atrial G protein-activated K+ channel: expression cloning and molecular properties.
    • 341
    • PDF
    Recombinant G-protein βγ-subunits activate the muscarinic-gated atrial potassium channel
    • 412
    Activation of the cloned muscarinic potassium channel by G protein βγ subunits
    • 446
    • Highly Influential
    Distinct sequence elements control the specificity of G protein activation by muscarinic acetylcholine receptor subtypes.
    • 154
    Charged amino acids required for signal transduction by the m3 muscarinic acetylcholine receptor.
    • 58