Identification of differentially expressed genes in Con A-activated carp (Cyprinus carpio L.) leucocytes.

@article{Yin1999IdentificationOD,
  title={Identification of differentially expressed genes in Con A-activated carp (Cyprinus carpio L.) leucocytes.},
  author={Zhongchao Yin and Jing He and Zhiyuan Gong and T. J. Lam and Yoke Min Sin},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={1999},
  volume={124 1},
  pages={41-50}
}
A cDNA library was constructed from the message RNA (mRNA) obtained from Con A-induced head kidney (HK) leucocytes of carp (Cyprinus carpio L.). Differential screening of the cDNA was carried out by hybridization against the total cDNA probes from normal, Con A-uninduced HK leucocytes or Con A-induced HK leucocytes of carp. The differential expression patterns of certain cDNA clones were confirmed by Southern-blot and Northern-blot analysis. Single-pass of the sequencing analysis and homology… CONTINUE READING

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