Identification of developmental regulatory genes in Aspergillus nidulans by overexpression.

@article{Marhoul1995IdentificationOD,
  title={Identification of developmental regulatory genes in Aspergillus nidulans by overexpression.},
  author={J F Marhoul and Thomas H. Adams},
  journal={Genetics},
  year={1995},
  volume={139 2},
  pages={537-47}
}
Overexpression of several Aspergillus nidulans developmental regulatory genes has been shown to cause growth inhibition and development at inappropriate times. We set out to identify previously unknown developmental regulators by constructing a nutritionally inducible A. nidulans expression library containing small, random genomic DNA fragments inserted next to the alcA promoter [alcA(p)] in an A. nidulans transformation vector. Among 20,000 transformants containing random alcA(p) genomic DNA… CONTINUE READING