Identification of detergent-resistant plasma membrane microdomains in dictyostelium: enrichment of signal transduction proteins.

@article{Xiao1997IdentificationOD,
  title={Identification of detergent-resistant plasma membrane microdomains in dictyostelium: enrichment of signal transduction proteins.},
  author={Zhan Xiao and Peter N. Devreotes},
  journal={Molecular biology of the cell},
  year={1997},
  volume={8 5},
  pages={855-69}
}
Unlike most other cellular proteins, the chemoattractant receptor, cAR1, of Dictyostelium is resistant to extraction by the zwitterionic detergent, CHAPS. We exploited this property to isolate a subcellular fraction highly enriched in cAR1 by flotation of CHAPS lysates of cells in sucrose density gradients. Immunogold electron microscopy studies revealed a homogeneous preparation of membrane bilayer sheets. This preparation, designated CHAPS-insoluble floating fraction (CHIEF), also contained a… CONTINUE READING
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