Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope

  title={Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope},
  author={Eva Ad{\'a}m and Stephen A. Adam},
  journal={The Journal of Cell Biology},
  pages={547 - 555}
Nuclear protein import can be separated into two distinct steps: binding to the nuclear pore complex followed by translocation to the nuclear interior. A previously identified nuclear location sequence (NLS) receptor and a 97-kD protein purified from bovine erythrocytes reconstitute the binding step in a permeabilized cell assay. Binding to the envelope is specific for a functional SV-40 large T antigen NLS and is not ATP or temperature dependent. Modification of p97 with N-ethylmaleimide (NEM… CONTINUE READING
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