Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora

  title={Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora},
  author={R. Nakamura and R. Kondo and Ming-hao Shen and H. Ochiai and S. Hisamatsu and Shigenori Sonoki},
  journal={AMB Express},
  pages={8 - 8}
  • R. Nakamura, R. Kondo, +3 authors Shigenori Sonoki
  • Published 2011
  • Biology, Medicine
  • AMB Express
  • Three cytochrome P450 monooxygenase (CYP) genes, designated pb-1, pb-2 and pb-3, were isolated from the white-rot fungus, Phlebia brevispora, using reverse transcription PCR with degenerate primers constructed based on the consensus amino acid sequence of eukaryotic CYPs in the O2-binding, meander and heme-binding regions. Individual full-length CYP cDNAs were cloned and sequenced, and the relative nucleotide sequence similarity of pb-1 (1788 bp), pb-2 (1881 bp) and pb-3 (1791 bp) was more than… CONTINUE READING
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