• DOI:10.1186/2191-0855-2-8
Corpus ID: 7212267

Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora

@article{Nakamura2011IdentificationOC,
  title={Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora},
  author={R. Nakamura and R. Kondo and Ming-hao Shen and H. Ochiai and S. Hisamatsu and Shigenori Sonoki},
  journal={AMB Express},
  year={2011},
  volume={2},
  pages={8 - 8}
}

R. Nakamura, R. Kondo, +3 authors Shigenori Sonoki

Published 2011

Biology, Medicine

AMB Express

Three cytochrome P450 monooxygenase (CYP) genes, designated pb-1, pb-2 and pb-3, were isolated from the white-rot fungus, Phlebia brevispora, using reverse transcription PCR with degenerate primers constructed based on the consensus amino acid sequence of eukaryotic CYPs in the O2-binding, meander and heme-binding regions. Individual full-length CYP cDNAs were cloned and sequenced, and the relative nucleotide sequence similarity of pb-1 (1788 bp), pb-2 (1881 bp) and pb-3 (1791 bp) was more than… CONTINUE READING

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