Identification of cysteine sulfenic acid in AhpC of alkyl hydroperoxide reductase.

  title={Identification of cysteine sulfenic acid in AhpC of alkyl hydroperoxide reductase.},
  author={Leslie B. Poole and Holly R Ellis},
  journal={Methods in enzymology},
C165S AhpC in its sulfenate (Cys-SO-) and presumed thiolate (Cys-S-) forms at pH 7 (pKa for sulfenic acid about pH 6.1) exhibit low extinction absorbance bands around 367 and 324 nm, respectively. Sulfenic acid content of the protein can be assessed by its reactivity with the chromophoric TNB anion. Using this technique, H2O2 titrations of C165S AhpC give a maximum of about 1 SOH per subunit on addition of 1.0 to 1.2 equivalents of H2O2. Cys46-SO- is moderately air stable at neutral pH and room… CONTINUE READING


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