Identification of cyclophilin as the erythrocyte ciclosporin-binding protein.

  title={Identification of cyclophilin as the erythrocyte ciclosporin-binding protein.},
  author={Brian Michael J Foxwell and GJ. Frazer and Mark Winters and Peter C Hiestand and R O Wenger and Bernhard Ryffel},
  journal={Biochimica et biophysica acta},
  volume={938 3},
Previous studies on the distribution of circulating ciclosporin have shown that the majority of the drug is associated with erythrocytes. In order to investigate the nature of ciclosporin-erythrocyte binding, binding studies were performed on isolated erythrocytes. At therapeutic concentrations (approx. 0.5 microgram/ml in whole blood) greater than 90% of the erythrocyte associated ciclosporin was found in the cytosol. The cytosolic binding capacity was approximately (2-2.5).10(5) molecules of… CONTINUE READING