Identification of conserved prolyl residue important for transport activity and the substrate specificity range of yeast plasma membrane Na+/H+ antiporters.

@article{KinclovZimmermannov2005IdentificationOC,
  title={Identification of conserved prolyl residue important for transport activity and the substrate specificity range of yeast plasma membrane Na+/H+ antiporters.},
  author={Olga Kinclov{\'a}-Zimmermannov{\'a} and Martin Zavrel and Hana Sychrov{\'a}},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 34},
  pages={30638-47}
}
Yeast plasma membrane Na+/H+ antiporters are divided according to their substrate specificity in two distinct subfamilies. To identify amino acid residues responsible for substrate specificity determination (recognition of K+), the Zygosaccharomyces rouxii Sod2-22 antiporter (non-transporting K+) was mutagenized and a collection of ZrSod2-22 mutants that improved the KCl tolerance of a salt-sensitive Saccharomyces cerevisiae strain was isolated. Several independent ZrSod2-22 mutated alleles… CONTINUE READING

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