Identification of cold shock gene loci in Sinorhizobium meliloti by using a luxAB reporter transposon.

@article{Oconnell2000IdentificationOC,
  title={Identification of cold shock gene loci in Sinorhizobium meliloti by using a luxAB reporter transposon.},
  author={K P O'connell and Ann Marie Gustafson and Mandy D. Lehmann and Michael F Thomashow},
  journal={Applied and environmental microbiology},
  year={2000},
  volume={66 1},
  pages={401-5}
}
Using a luxAB reporter transposon, seven mutants of Sinorhizobium meliloti were identified as containing insertions in four cold shock loci. LuxAB activity was strongly induced (25- to 160-fold) after a temperature shift from 30 to 15 degrees C. The transposon and flanking host DNA from each mutant was cloned, and the nucleic acid sequence of the insertion… CONTINUE READING