Identification of clinical staphylococcal isolates from humans by internal transcribed spacer PCR.

@article{Couto2001IdentificationOC,
  title={Identification of clinical staphylococcal isolates from humans by internal transcribed spacer PCR.},
  author={Isabel Couto and S{\'o}nia Pereira and Maria Miragaia and Ilda Santos Sanches and Herminia de Lencastre},
  journal={Journal of clinical microbiology},
  year={2001},
  volume={39 9},
  pages={3099-103}
}
The emergence of coagulase-negative staphylococci not only as human pathogens but also as reservoirs of antibiotic resistance determinants requires the deployment and development of methods for their rapid and reliable identification. Internal transcribed spacer-PCR (ITS-PCR) was used to identify a collection of 617 clinical staphylococcal isolates. The amplicons were resolved in high-resolution agarose gels and visually compared with the patterns obtained for the control strains of 29… CONTINUE READING

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