Identification of cellular proteins enhancing activities of internal ribosomal entry sites by competition with oligodeoxynucleotides.

@article{Choi2004IdentificationOC,
  title={Identification of cellular proteins enhancing activities of internal ribosomal entry sites by competition with oligodeoxynucleotides.},
  author={Kobong Choi and Jong Heon Kim and X Li and Ki Young Paek and Sang Hoon Ha and Sung Ho Ryu and Eckard J Wimmer and S. K. Jang},
  journal={Nucleic acids research},
  year={2004},
  volume={32 4},
  pages={1308-17}
}
The translation of numerous eukaryotic mRNAs is mediated by internal ribosomal entry sites (IRESs). IRES-dependent translation requires both canonical translation initiation factors and IRES-specific trans-acting factors (ITAFs). Here we report a strategy to identify and characterize ITAFs required for IRES-dependent translation. This process involves steps for identifying oligodeoxynucleotides affecting IRES-dependent translation, purifying proteins interacting with the inhibitory DNA… CONTINUE READING