Identification of calcium conducting channels in isolated boar sperm plasma membranes.


Ion channel recordings were obtained from liposomes containing purified boar sperm plasma membrane proteins using a tip-dip method. Liposomes prepared in HEPES-TRIS and clamped by electrodes containing Ba-HEPES displayed channel activity that was partially inhibited by verapamil or nitrendipine and completely inhibited by La3+. Reversal of current at pipette negative voltages was observed only when Ba2+ ions were also present in the bath solution. These data indicate that channels capable of carrying calcium currents are prominent components of the plasma membrane of mammalian sperm.


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@article{Cox1989IdentificationOC, title={Identification of calcium conducting channels in isolated boar sperm plasma membranes.}, author={Teresa Cox and Rachel N Peterson}, journal={Biochemical and biophysical research communications}, year={1989}, volume={161 1}, pages={162-8} }