Identification of caerulomycin A gene cluster implicates a tailoring amidohydrolase.


The biosynthetic gene cluster for caerulomycin A (1) was cloned and characterized from the marine actinomycete Actinoalloteichus cyanogriseus WH1-2216-6, which revealed an unusual hybrid polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) system. The crmL disruption mutant accumulated caerulomycin L (2) with an extended L-leucine at C-7, implicating an amidohydrolase activity for CrmL. The leucine-removing activity was confirmed for crude CrmL enzymes. Heterologous expression of the 1 gene cluster led to 1 production in Streptomyces coelicolor.

DOI: 10.1021/ol300589r


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@article{Zhu2012IdentificationOC, title={Identification of caerulomycin A gene cluster implicates a tailoring amidohydrolase.}, author={Yiguang Zhu and Peng Fu and Qinheng Lin and Guangtao Zhang and Haibo Zhang and Sumei Li and Jianhua Ju and Weiming Zhu and Changsheng Zhang}, journal={Organic letters}, year={2012}, volume={14 11}, pages={2666-9} }