Identification of cDNA clones that code for protein-tyrosine kinases by screening expression libraries with antibodies against phosphotyrosine.

@article{Lindberg1988IdentificationOC,
  title={Identification of cDNA clones that code for protein-tyrosine kinases by screening expression libraries with antibodies against phosphotyrosine.},
  author={Richard A. Lindberg and David P. Thompson and Tony Hunter},
  journal={Oncogene},
  year={1988},
  volume={3 6},
  pages={629-33}
}
A phage lambda gt11 human fibroblast cDNA expression library was screened with antibodies against phosphotyrosine to determine the feasibility of this approach as a method for the identification of clones that code for protein-tyrosine kinases. Several antibody positive clones were isolated. One clone also scored positive with degenerate oligonucleotides… CONTINUE READING