Identification of bovine Neospora parasites by PCR amplification and specific small-subunit rRNA sequence probe hybridization.

@article{Ho1996IdentificationOB,
  title={Identification of bovine Neospora parasites by PCR amplification and specific small-subunit rRNA sequence probe hybridization.},
  author={Matthew Sai Pong Ho and Bradd C. Barr and Antoinette E. Marsh and Mark L. Anderson and Joan Dean Rowe and Alice F. Tarantal and Andrew G. Hendrickx and Karen W Sverlow and Jitender Prakash Dubey and Patricia A. Conrad},
  journal={Journal of clinical microbiology},
  year={1996},
  volume={34 5},
  pages={1203-8}
}
Neospora is a newly recognized genus of pathogenic coccidia, closely related to Toxoplasma gondii, that can cause abortion or congenital disease in a variety of domestic animal hosts. On the basis of the small-subunit rRNA gene sequences of Neospora spp. and other apicomplexa coccidia, oligonucleotide primers COC-1 and COC-2 were used for PCR amplification of conserved sequences of approximately 300 bp in size. A Neospora-specific chemiluminescent probe hybridized to Southern blots of… CONTINUE READING

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