Identification of bloodmeals in haematophagous Diptera by cytochrome B heteroduplex analysis

  title={Identification of bloodmeals in haematophagous Diptera by cytochrome B heteroduplex analysis},
  author={Daniel A. Boakye and J M Tang and Philippe Truc and Anthony Merriweather and Thomas R. Unnasch},
  journal={Medical and Veterinary Entomology},
We developed a DNA assay for bloodmeal identification in haematophagous insects. [] Key Result (Diptera: Simuliidae), human cytochrome B DNA sequences were identifiable up to 3 days following ingestion of the bloodmeal. In the tsetse Glossina palpalis (Diptera: Glossinidae) collected from tsetse traps in Ivory Coast, bloodmeals were identified as taken from domestic pigs on the basis of their heteroduplex pattern and DNA sequence.

Identification of blood meals imbibed by phlebotomine sand flies using cytochrome b PCR and reverse line blotting.

A vertebrate-specific PCR is combined with reverse line blot analysis for identifying blood meals ingested by female phlebotomine sand fly vectors of leishmaniasis, and the source of blood was identified in 68 of 89 wild-caught sand flies tested.

Combination of cytochrome b heteroduplex-assay and sequencing for identification of triatomine blood meals.

  • R. BuitragoS. Depickère S. Brenière
  • Biology
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
  • 2012

Molecular Identification of Bloodmeal Source in Ixodes ricinus Ticks Using 12S rDNA As a Genetic Marker

An efficient molecular method was developed for the identification of the bloodmeal sources in the tick Ixodes ricinus (L), the European vector of the agents of Lyme borreliosis and tick-borne encephalitis, and to determine the source of infective bloodmeal and to identify reservoir species.

Molecular identification of blood meal sources of ticks (Acari, Ixodidae) using cytochrome b gene as a genetic marker

In this study, Cyt b gene as a molecular target produced reliable results and was very significant for the effective identification of ticks’ blood meal.

Identifying the last supper: utility of the DNA barcode library for bloodmeal identification in ticks

The utility of the DNA barcode library is validates as a valuable and reliable resource for the identification of unknown bloodmeals in arthropod vectors of disease.

Phlebotomine (Diptera, Psychodidae) Bloodmeal Sources in Tunisian Cutaneous Leishmaniasis Foci: Could Sergentomyia minuta, which is not an Exclusive Herpetophilic Species, be Implicated in the Transmission of Pathogens?

Interestingly, it is found for the first time that Mus musculus DNA was found in one female of S. minuta (Rondani) specie and question about its possible vectorial role is opened.

Identification of Mosquito Bloodmeals Using Polymerase Chain Reaction (PCR) With Order-Specific Primers

A polymerase chain reaction (PCR) protocol was developed to identify host bloodmeals from mosquitoes and American crows were distinguished from other passeriformes by restriction enzyme digestion.

Identification in triatomine vectors of feeding sources and Trypanosoma cruzi variants by heteroduplex assay and a multiplex miniexon polymerase chain reaction.

Feeding sources of triatomine vectors (Triatoma longipennis) collected in peridomiciles in Mexico were identified by a heteroduplex assay developed with triatomines blood meals, and Trypanosoma cruzi was the only flagellate species identified in the blood meals.



A molecular marker for the identification of the zoonotic reservoirs of Lyme borreliosis by analysis of the blood meal in its European vector Ixodes ricinus

The efficacy of the mitochondrially encoded cytochrome b gene as a molecular marker for the discrimination of the reservoir host species of the Lyme borreliosis spirochete, Borrelia burgdorferi sensu

ELISA for identification of blood meal source in black flies (Diptera: Simuliidae).

A modified ELISA, in which a biotinylated second antibody and a streptavidin-biotinylated peroxidase complex are used, has been developed to identify the blood meal sources of black flies. The

Analysis of mosquito bloodmeals by DNA profiling

Variable numbers of tandem repeat (VNTR) sequences are employed to prime amplification of human DNA in the polymerase chain reaction (PCR) and the radiolabeled products are analysed by high resolution denaturing gel electrophoresis for identification of the individual human host.

Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers.

The polymerase chain reaction is used to amplify homologous segments of mtDNA from more than 100 animal species, including mammals, birds, amphibians, fishes, and some invertebrates, and the unexpectedly wide taxonomic utility of these primers offers opportunities for phylogenetic and population research.

Species identification of blood-meals from tsetse flies (Glossinidae): results 1979-1985.

  • C. StaakU. KämpeG. Korkowski
  • Biology, Medicine
    Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit
  • 1986
It was shown that the preference of tsetse flies for certain host species differed according to the sampling area, and results from blood-meal identification are to be interpreted with this point in view.

FINS (forensically informative nucleotide sequencing): A procedure for identifying the animal origin of biological specimens.

FINS is a rapid, reliable and reproducible procedure that is based on established techniques that fills the need for an accurate method of determining the species identity of a specimen when this is not possible by conventional means.

Identification of blood meals in Aedes aegypti by antibody sandwich enzyme-linked immunosorbent assay.

The sandwich-B ELISA assay was superior to the direct assay and was thus selected as the method of choice for future field studies.

Technical report. Optimizing probe selection in directed heteroduplex analysis using HDprobe 1.1.

A computer program has been developed that predicts the number of potential stable and unstable mismatches between a probe and its target sequences in DHDA to help identify informative probes in the development of new DHDA-based assays.

A new simple technique for rearing F1 progeny from females of the Simulium damnosum Theobald complex.

A simple, readily transportable rearing apparatus was developed for investigations by the Onchocerciasis Control programme by using unbreakable, easily packed and reassembled parts and feeding the larvae at least partly with living green algae cultured in the laboratory.