Identification of biliary metabolites of (-)-epigallocatechin gallate in rats.

  title={Identification of biliary metabolites of (-)-epigallocatechin gallate in rats.},
  author={Kazuaki Kida and Masayuki Suzuki and Naoki Matsumoto and Fumio Nanjo and Yukihiko Hara},
  journal={Journal of agricultural and food chemistry},
  volume={48 9},
After oral administration of (-)-epigallocatechin gallate (EGCg) to rats, its biliary metabolites were examined. Although a large part of the biliary metabolites was found to exist in conjugated forms, it was difficult to separate the conjugated forms. Thus the free form of biliary metabolites was prepared by beta-glucuronidase/sulfatase treatment and was purified by HPLC. Six compounds purified were subjected to FABeta-MS and NMR analyses. The six metabolites thus obtained were shown to be… CONTINUE READING