Identification of beta subunit of the rhesus monkey chorionic gonadotropin (rmCG)


Chorionic gonadotropin (CG) is a placental derived hormone that plays a crucial role in successful implantation and establishment of early pregnancy in the primates. The rhesus monkey was chosen as a model to understand the feasibility of developing human DNA immuno-contraceptive. The coding region of rhesus monkey CG β-subunit (rmCGβ) was isolated by the TDRT-PCR method. The nucleotide sequence including the leader peptide was 499 nucleotide long and encoded 166 amino acids. In comparing with the previous known primates CG β-subunits, the rmCGβ was the highest degree of homology with baboon CG β-subunit at the deduced amino acid sequence (94%), 79.5% homology with human CG β-subunit and 70.4% homology with marmoset monkey CG β-subunit. The eukaryotic expression vector pCMV4-rmCGβ inserted full-coding cDNA sequence of rmCGβ was constructed, and the expression of rmCG β-subunit in HeLa cells transient expressing system in vitro and BALB/c mice in vivo was determined. The results demonstrated that the recombinant PCMV4-rmCGβ eukaryotic expression vector could express rmCG β-subunit in vitro and in vivo.

DOI: 10.1023/A:1007233329355

8 Figures and Tables


Citations per Year

201 Citations

Semantic Scholar estimates that this publication has 201 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Chen2001IdentificationOB, title={Identification of beta subunit of the rhesus monkey chorionic gonadotropin (rmCG)}, author={Yun Chen and Zhe Liu and Bin Wang and Jing-pian Peng}, journal={Molecular and Cellular Biochemistry}, year={2001}, volume={218}, pages={157-163} }