Identification of base-specific contacts in protein-DNA complexes by photocrosslinking and mass spectrometry: a case study using the restriction endonuclease SsoII.

@article{Pingoud2005IdentificationOB,
  title={Identification of base-specific contacts in protein-DNA complexes by photocrosslinking and mass spectrometry: a case study using the restriction endonuclease SsoII.},
  author={Vera Pingoud and Hildegard Geyer and Rudolf Geyer and Elena Kubareva and Janusz M. Bujnicki and Alfred Pingoud},
  journal={Molecular bioSystems},
  year={2005},
  volume={1 2},
  pages={135-41}
}
Specific protein-nucleic acid interactions are of paramount importance for the propagation, maintenance and expression of genetic information. Restriction endonucleases serve as model systems to study the mechanisms of DNA recognition by proteins. SsoII is a Type II restriction endonuclease that recognizes the double stranded sequence downward arrow CCNGG and cleaves it in the presence of Mg(2+)-ions, as indicated. SsoII shows sequence similarity over a stretch of approximately 70 amino acid… CONTINUE READING